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Lake Ice ResearchThe Science Jar (tm) has started a new research project on 11 Feb 2023 to assess the microorganism population to be found in lake ice. Ice on the surface of the lake, near the edge, was broken up and small pieces collected into a cooler at around 3pm. A sample of water was also syringed out from near the bottom. Of the ice samples, to assure that no microorganisms were carried from water droplets on them were washed with tap water before being placed into a glass jar also pre-washed out with tap water and dried with a paper towel. The washed ice samples were placed in the larger of the two jars. Unwashed ice samples were put into a smaller glass jar. The syringed out water sample from near the bottom was placed in a small sterile plastic container. Each of the ice samples were allowed to thaw at room temperature.  Location from which Ice & Water Samples Obtained  Ice Samples placed in cooler for Transport  Some Ice Samples Washed with Tap Water to Make Sure Microorganisms in Sample From Within Ice Not Within Water Droplets On the Ice   5 Hours After Collection; 8pm The jars were not completely thawed yet however I took 2 8 ML samplings from the washed ice jar. The first one I spun down at 1000rpm for a couple minutes and the second I spun down at 2000rpm for a couple minutes. In neither sample was there any movement detected whatsoever. There is algae in there and some interesting amber colored filaments at this point. After the samplings I placed all of the samples beside The Science Jar under the grow light. |
The Water Sample Syringed from Bottom of Litteral Zone at Edge of Lake Below where Ice Samples were Taken From  
 1 Hour After Collection; 4pm  2 Hours After Collection; 5pm  3 Hours After Collection; 6pm  4 Hours After Collection; 7pm |